Ammonia Nitrogen Test

High Range
Test N Tube
0-50 mg/L NH3-N
Salicylate Method

1. Press : PRGM
2. Press : 67 ENTER
The display will show mg/L, NH3-N & Zero icon.
3. Insert the COD/TNT Adapter into the cell holder by rotating the adapter until it drops into place. Then push down to fully insert it.
4. Remove the caps from 2Am Ver Diluent Reagent High Range Vials. Add 0.1 ml of sample to one vial (the sample). Add 0.1 ml of DI water to the other (BLANK).
5. Add the contents of 1 Ammonia Salicylate Reagent Powder Pillow for 5 ml sample to each vial.
6. Add the contents of 1 Ammonia Cyanurate Reagent Powder Pillow for 5 ml sample to each vial.
7. Cap the vials tightly and snake thoroughly to dissolve the powder.
Note : A green color will develop if ammonia is present.
8. Press : TIMER ENTER
A 20 minute reaction period will begin.
9. Clean the outside of the vial with a towel . After the timer beeps,  place the blank into the vial adapter. Tightly cover the instrument cap.
10. Press : ZERO
11. Place the prepared sample in the adaptor.
Push Straight down on the top of the vial until it seats solidly into the adapter
12. Tightly cover the vial with the instrument cap.
13. Press : READ

Storage of Samples

Most reliable results are obtained on fresh samples.
If samples are to be analyzed within 24 h of collection, refrigerate unacidified at 4°C.
For preservation for up to 28 d, freeze at  − 20°C unacidified, or preserve samples by a  cidifying to pH <2 and storing at 4°C.

CAUTION:
If acid preservation is used, neutralize samples with NaOH or KOH immediately before making the determination. 
Although acidification is suitable for certain types of samples, it produces interferences when e xchangeable ammonium is present in unfiltered solids.